Legalizing Marijuana Research Paper Example | Topics and Well Written Essays - 1500 words

Legalizing Marijuana - Research Paper Example It is also believed that India was one of the few countries which pioneered the consumption of this plant as a tool for recreation and relaxation. The Sadhus and the Gurus who practiced the art of spiritual elevation found this plant to be extremely useful for their purpose. They gained a lot from its usage whereby it was considered to be a holy drug. Earlier texts of Hinduism dictate that Marijuana was used by the Hindu Gods to evolve and to relax at the same time. (ElSohly, Mahmoud) Ancient Hindu text Soma Veda refers to Marijuana as the â€Å"food of the thinking mind.† Indians call it Ganja. It has served millions of people over a period of time and has been acclaimed as a life saving drug. However, there are side effects of this plant and if consumed by the innocent it may drive one to an extremely negative frame of mind. Marijuana comes from the plant known as Cannabis Sativa. The major psychoactive compound in the plant is known as THC, and this is the element which is responsible for the psychoactive effects in the human mind. The female plant of Cannabis Sativa is consumed by people as it is said to be more potent with THC than the male plant. (Rudgley, Richard) Marijuana has been used for medicinal as well as recreational purposes. Marijuana influences the human body and mind in many different ways. It has the power of changing the mood to the perception of the human mind for a particular object. It enhances hunger when consumed and can lead to weight loss among those who consume it. It relaxes the muscles and also helps the mind calm down after the THC molecules hit the ‘cannabinoid’ receptors which are present in the human mind. The situation for the usage of Marijuana remains static as most of the countries have banned the recreational use of this drug, however, it is allowed in those countries to be used for medicinal purposes. (Gettman, Jon) For those who have tried and are not prejudiced towards it, it is

Dirty Water Bill Assignment Example | Topics and Well Written Essays - 250 words

Dirty Water Bill - Assignment Example This may lead to more damage downstream as these pollutants begin to accumulate. This policy as stated in the public outcry is literally similar to the notion of whatever happens in Vegas should stay in Vegas so this policy would fail quickly (Carter, National Wildlife Federation). H.R. 2018 bill was likely to threaten water quality in American rivers, streams, and lakes. The ability of federal and state governments would be limited in terms of ensuring that all American citizens can access clean water. The bill would limit the Environmental Protection Agency’s capability of revising an agreed water quality standard; this would happen only if the state agrees. It would prohibit the EPA from disallowing a water quality certification that is granted by a state. It would hinder the EPA from limiting federal financial aid for the state water quality program if a state does not comply with the water quality standards. Protected waters, aquatic waters would become degraded since the H.R. Bill 2018 renders EPA as somewhat meaningless. This includes weakening permit reviews that normally determine alternatives that do not harm the environment, do not go against laws, and do not degrade waters. H.R. Bill 2018 would lead to harm to the environment in the short ru n and long run. United States. Congress. House. Committee on Rules. Providing for Consideration of the Bill (H.R. 2018) to Amend the Federal Water Pollution Control Act to Preserve the Authority of Each State to Make Determinations Relating to the States Water Quality Standards, and for Other Purposes: (to Accompany H. Res. 347). Washington: U.S. Government Printing Office, 2011.

Compare the scandal involving President Clinton with Nixon's Research Paper

Compare the scandal involving President Clinton with Nixon's Watergate. What were the key similarities and differences - Research Paper Example In June 1995, a scandal began during the latter half of his second term involving Monica Lewinsky, 21, who came to the White House as an unpaid intern in the office of White House Chief of Staff, Leon Panetta. In November 1995, she got involved in a sexual relationship with President Clinton. Barely a month into the relationship, Lewinsky was moved to a paid position in the Office of Legislative Affairs, handling letters from members of Congress. She frequently ferried mail to the Oval Office. Around April, 1996, Lewinsky was transferred to a job as an assistant to Pentagon spokesman Ken Bacon, by Evelyn Lieberman who was then the Deputy White House Chief of Staff. Lieberman told the New York Times later that the move was due to â€Å"inappropriate and immature behavior† and that Lewinsky demonstrated a lack of attention to work. At the Pentagon, Lewinsky met Linda Tripp, a career government employee. Around summer 1996, Lewinsky began telling Tripp during casual conversations about her relationship with President Clinton. Tripp reported these conversations to literary agent Lucianne Goldberg, who advised her to secretly record them. Tripp convinced Lewinsky to save the gifts she received from the President and the dress that got stained during one of her sexual encounters. About a year later, in the fall of 1997, when the relationship was over, Tripp secretly began taping the conversations with Lewinsky in which she explicitly talked about her alleged affair. Around October 1997, Tripp, with help from Lucianne Goldberg, met with Newsweek’s Michael Isikoff, Kucianne & Jonah Goldberg at Jonah’s apartment in Washington, where they listened to a tape with the Tripp/Lewinsky conversation. In October 1997, Lewinsky interviewed with U.S. Ambassador to the U.N., Bill Richardson for a low level public affairs position in New York. She was offered a job but she declined. In early December that year, Lewinsky left the Pentagon. Later that month, Bett ie Currie, President Clinton’s personal secretary, got in touch with Vernon Jordan, a friend of Clintons, to place Lewinsky in New York. Vernon Jordan obliged and introduced Lewinsky to several leads. Lewinsky allegedly had over 9 sexual encounters with the President in the Oval Office during the period between November 1995 and March 1997. On December 17, 1997, Lewinsky was subpoenaed by lawyers for Paula Jones, who was suing the President on sexual harassment charges. She submitted an affidavit in which she declared that she never had any sexual relationship with the President. She also, allegedly attempted to persuade Tripp to lie under oath in the Jones case. Tripp, instead, gave the tapes to Kenneth Starr, who was the Independent Counsel investigating the Whitewater & Paula Jones case. Lewinsky made her final visit to the White House on December 28, 1997, when she and the President had a private meeting. During this meeting, the President allegedly encouraged Lewinsky to be as evasive as possible in her responses to any of the questions in the Jones’ lawsuit. On January 16th 1998, Kenneth Starr contacted United States Attorney General, Janet Reno, seeking permission to expand his probe to investigate possible impropriety on the part of the President. The Attorney General gave her consent and submitted a request to a panel of three Federal judges. The judges agreed to allow Kenneth Starr to formally investigate the possibility of subornation of perjury and obstruction of justice

Introduce the two companies and explain the difference between a b2b Essay

Introduce the two companies and explain the difference between a b2b company and a b2c company - Essay Example is a B2B company since it provides manufactures and supplies microprocessors necessary for computer manufacturing companies such as Dell, HP, Samsung, Sony etc. On the other hand Dell is a B2C company since the products manufactured by this company is sold directly to the consumers. B2B and B2C companies differ in terms of marketing mix, product ranges, marketing communication, pricing and distribution Product, Price, Place and Promotion are considered as the four major elements of marketing mix (Ritcher, 2012). Intel is one of the major semiconductor chip manufacturers in the world. It supplies many types of semiconductor chips including microprocessors to prominent computer manufacturers as well as electronic devices manufacturers. Therefore, the company is targeting only the major manufacturers of electronic devices such as Dell, Sony, HP, Lenovo etc. Unlike B2C companies, B2B companies or Intel is operating through channel partners in different market. For example, LG, Lenovo, Dell and Zenith are some of the channel partners of Intel in different markets (Mody, 2007). Intel has a variable pricing strategy across the world. Since the company enjoys monopoly in some markets, it has the ability to fix the prices of its products in such markets. As in the case of B2C companies, Intel also use various promotion strategies to get more market share. â€Å"In 2006 Intel launched a fi ve-year campaign known as the ‘World Ahead Program’. This initiative is geared toward an increase in digital literacy, research, and development†(Flores, 2008). Dell on the other hand has a wide range of products such as workstations, desktops, desktops, tablets, etc. The company is marketing its products directly to the consumers with the help of advertising and promotion strategies. Promotion plays a big part in Dell’s marketing mix even though it plays less significant role in Intel’s marketing mix. Variable pricing mechanisms were implemented by dell in different markets based

Compare the scandal involving President Clinton with Nixon's Research Paper

Compare the scandal involving President Clinton with Nixon's Watergate. What were the key similarities and differences - Research Paper Example In June 1995, a scandal began during the latter half of his second term involving Monica Lewinsky, 21, who came to the White House as an unpaid intern in the office of White House Chief of Staff, Leon Panetta. In November 1995, she got involved in a sexual relationship with President Clinton. Barely a month into the relationship, Lewinsky was moved to a paid position in the Office of Legislative Affairs, handling letters from members of Congress. She frequently ferried mail to the Oval Office. Around April, 1996, Lewinsky was transferred to a job as an assistant to Pentagon spokesman Ken Bacon, by Evelyn Lieberman who was then the Deputy White House Chief of Staff. Lieberman told the New York Times later that the move was due to â€Å"inappropriate and immature behavior† and that Lewinsky demonstrated a lack of attention to work. At the Pentagon, Lewinsky met Linda Tripp, a career government employee. Around summer 1996, Lewinsky began telling Tripp during casual conversations about her relationship with President Clinton. Tripp reported these conversations to literary agent Lucianne Goldberg, who advised her to secretly record them. Tripp convinced Lewinsky to save the gifts she received from the President and the dress that got stained during one of her sexual encounters. About a year later, in the fall of 1997, when the relationship was over, Tripp secretly began taping the conversations with Lewinsky in which she explicitly talked about her alleged affair. Around October 1997, Tripp, with help from Lucianne Goldberg, met with Newsweek’s Michael Isikoff, Kucianne & Jonah Goldberg at Jonah’s apartment in Washington, where they listened to a tape with the Tripp/Lewinsky conversation. In October 1997, Lewinsky interviewed with U.S. Ambassador to the U.N., Bill Richardson for a low level public affairs position in New York. She was offered a job but she declined. In early December that year, Lewinsky left the Pentagon. Later that month, Bett ie Currie, President Clinton’s personal secretary, got in touch with Vernon Jordan, a friend of Clintons, to place Lewinsky in New York. Vernon Jordan obliged and introduced Lewinsky to several leads. Lewinsky allegedly had over 9 sexual encounters with the President in the Oval Office during the period between November 1995 and March 1997. On December 17, 1997, Lewinsky was subpoenaed by lawyers for Paula Jones, who was suing the President on sexual harassment charges. She submitted an affidavit in which she declared that she never had any sexual relationship with the President. She also, allegedly attempted to persuade Tripp to lie under oath in the Jones case. Tripp, instead, gave the tapes to Kenneth Starr, who was the Independent Counsel investigating the Whitewater & Paula Jones case. Lewinsky made her final visit to the White House on December 28, 1997, when she and the President had a private meeting. During this meeting, the President allegedly encouraged Lewinsky to be as evasive as possible in her responses to any of the questions in the Jones’ lawsuit. On January 16th 1998, Kenneth Starr contacted United States Attorney General, Janet Reno, seeking permission to expand his probe to investigate possible impropriety on the part of the President. The Attorney General gave her consent and submitted a request to a panel of three Federal judges. The judges agreed to allow Kenneth Starr to formally investigate the possibility of subornation of perjury and obstruction of justice

The companys clothing and shoe designs typically feature Essay Example for Free

The companys clothing and shoe designs typically feature Essay Adidas was founded in 1948 by Adolf Dassler, following the split of Gebrà ¼der Dassler Schuhfabrik between him and his older brother Rudolf. Rudolf later established Puma, which was the early rival of Adidas. Registered in 1949, Adidas is currently based in Herzogenaurach, Germany. Puma is also based in Herzogenaurach. The companys clothing and shoe designs typically feature three parallel bars, and the same motif is incorporated into Adidass current official logo.The company revenue for 2012 was listed at â‚ ¬14.48 billion. History Gebrà ¼der Dassler Schuhfabrik Christoph Von Wilhelm Dassler was a worker in a shoe factory, while his wife Pauline ran a small laundry in the Bavarian town of Herzogenaurach, 20 km (12. 4 mi) from the city of Nuremberg. After leaving school, their son, Rudolf Rudi Dassler, joined his father at the shoe factory. When he returned from fighting in World War I, Rudolf received a management position at a porcelain factory, and later in a leather wholesale business in Nuremberg. Adolf Adi Dassler started to produce his own sports shoes in his mothers wash kitchen in Herzogenaurach, Bavaria after his return from World War I. In July 1924, his brother Rudolf returned to Herzogenaurach to join his younger brothers business, which became Gebrà ¼der Dassler Schuhfabrik (Dassler Brothers Shoe Factory) and prospered. The pair started the venture in their mothers laundry,[6]:5 but, at the time, electricity supplies in the town were unreliable, and the brothers sometimes had to use pedal power from a stationary bicycle to run their equipment. By the 1936 Summer Olympics, Adi Dassler drove from Bavaria on one of the worlds first motorways to the Olympic village with a suitcase full of spikes and persuaded U.S. sprinter Jesse Owens to use them, the first sponsorship for an African American. Following Owenss haul of four gold medals, his success cemented the good reputation of Dassler shoes among the worlds most famous sportsmen. Letters from around the world landed on the brothers desks, and the trainers of other national teams were all interested in their shoes. Business boomed and the Dasslers were selling 200,000 pairs of shoes each year before World War II. World War II and company split Both brothers joined the Nazi Party, but Rudolf was slightly closer to the party than Adolf. During the war, a growing rift between the pair reached a breaking point after an Allied bomb attack in 1943, when Adi and his wife climbed into a bomb shelter that Rudolf and his family were already in: The dirty bastards are back again, Adi said, referring to the Allied war planes, but Rudolf was convinced his brother meant him and his family.[9] After Rudolf was later picked up by American soldiers and accused of being a member of the Waffen SS, he was convinced that his brother had turned him in. The Dassler factory, used for production of anti-tank weapons during the war, was nearly destroyed by US forces in April 1945, but was spared when Adi Dasslers wife, Kà ¤the, convinced the GIs that the company and its employees were only interested in manufacturing sports shoes. American occupying forces subsequently became major buyers of the Dassler brothers shoes. The brothers split up in 1947, with Rudi forming a new firm that he called Ruda – from Rudolf Dassler, later rebranded Puma, and Adi forming a company formally registered as Adidas AG from Adi Dassler on 18 August 1949. Although it is popularly claimed that the name is an acronym for All Day I Dream About Soccer, that phrase is a backronym; the name is actually a portmanteau formed from Adi (a nickname for Adolf) and Das (from Dassler). Early years and rivalry with Puma Puma and Adidas entered a fierce and bitter rivalry after the split. The town of Herzogenaurach was divided on the issue, leading to the nickname the town of bent necks—people looked down to see which shoes strangers wore. Even the towns two football clubs were divided: ASV Herzogenaurach club supported Adidas, while 1 FC Herzogenaurach endorsed Rudolfs footwear. When handymen were called to Rudolfs home, they would deliberately wear Adidas shoes. Rudolf would tell them to go to the basement and pick out a pair of free Pumas.The two brothers never reconciled, and although both are buried in the same cemetery, they are spaced apart as far as possible.[citation needed] In 1948, the first football match after World War II, several members of the West German national football team wore Puma boots, including the scorer of West Germanys first post-war goal, Herbert Burdenski. Four years later, at the 1952 Summer Olympics, 1500 metres runner Josy Barthel of Luxembourg won Pumas first Olympic gold in Helsinki, Finland. The original Adidas logo until 1997, it is now used on Adidas Originals At the 1960 Summer Olympics Puma paid German sprinter Armin Hary to wear Pumas in the 100 metre sprint final. Hary had worn Adidas before and asked Adolf for payment, but Adidas rejected this request. The German won gold in Pumas, but then laced up Adidas for the medals ceremony, to the shock of the two Dassler brothers. Hary hoped to cash in from both, but Adi was so enraged he banned the Olympic champion.

Purification of 1,3-PD from Complex Fermentation Broth

Purification of 1,3-PD from Complex Fermentation Broth MTZ is calculated as Eq. 6[24], where H is adsorption bed height (cm). tb is the time (min) of breakthrough point, which defined as the time when Ct/C0=0.05. te is the time (min) of saturated point, which defined as the time when Ct/C0=0.95. As showed in Table 2, increasing the 1,3-PD concentration, tb and te decreased. The dynamic adsorption of 1,3-PD to resin usually fails to achieve adsorption equilibrium. When the concentration of 1,3-PD achieved a certain high value, the breakthrough point and saturated point came early. The adsorption process in a fixed bed for a single adsorbate can be classified into three zones: the saturated zone, mass transfer zone and fresh zone[24]. The smaller the mass transfer zone and fresh zone were, the higher efficiency of the adsorption process. When the concentration of 1,3-PD was 35.2 g L-1, the maximum value of mass transfer zone (MTZ) is 23.2 cm, which indicated that the saturated zone was small and the efficiency was low. On the contrary, when the concentration of 1,3-PD was 25.0 g L-1, the MTZ was minimum while the efficiency of adsorption was 63.5% as the highest point. However, the production efficiency was lower for saturated time delayed. The breakthrough curves of glycerol were showed in Fig. 3B with three concentrations of 1,3-PD. When 1,3-PD attained saturated point, glycerol did not saturate and the adsorption is 428, 388 and 266 mg/g, respectively, which indicated the decreasing of the fermentation broth concentration increased the adsorption of glycerol. Considering both adsorption rate and efficiency, fermentation broth with 30.0 g L-1 concentrations were used in the following experiments. Data was fitted based on Yoon-Nelson model, which was showed in Fig. 4. Simulated parameters were listed in Table 2. The results indicated that k increased with the 1,3-PD concentration, while xexp decreased, which indicated that increasing concentration shortened the saturated time in the adsorption bed. Furthermore, xexp was similar with x, which indicated that Yoon-Nelson model can be applied to simulate the adsorption process with different concentrations. 3.3 The effect of flow rate The effect of flow rate to the adsorption of 1,3-PD was showed in Fig. 5A. From Fig. 5A, increasing the flow rate lead to the increasing of curves slope, the breakthrough time was shortened and the changing of the concentration difference was quicker. The main reason was that with the increasing of flow rate, exchanging time of adsorbate and resin was shortened. Further analysis showed in Table 3. From Table 3, with the setting up of flow rate, the saturated point of 1,3-PD decreased quickly and MTZ increased. High flow rate would make it difficult for 1,3-PD to transfer into resin porosity, which went against for the mass transfer of 1,3-PD and the undisturbed 1,3-PD increased. When the flow rate was 0.800 mL min-1, the adsorption rate was 62.2%. However, low flow rate lead to the delay of 1,3-PD saturated point and low adsorption rate, which is not benefit for industrial production. Data fitting based on Yoon-Nelson kinetic model, the result of 1,3-PD breakthrough curves showed in Table 3 and Fig. 6. As showed in Fig. 6, k increased with the increasing flow rate and xexp shared a similarity with x, indicating that Yoon-Nelson kinetic model can imitate adsorption process at different flow rates. 3.4 The effect of temperature The effect of temperature in the adsorption of 1,3-PD showed in Fig. 7A. As showed in Fig. 7A, tb and te increased with temperature. The breakthrough point delayed, which was similar to the result of a steady state adsorption. Increasing temperature promotes the adsorption of 1,3-PD to resin. However, the effect of temperature on breakthrough curves is not obvious on the slope of three curves was almost equal. The breakthrough curves of glycerol were shown in Fig. 7B. When the adsorption of 1,3-PD achieved saturate point, glycerol did not saturate and the adsorption is 310, 388 and 433 mg/g, respectively. Additional analysis was showed in Table 4. Although increasing temperature is beneficial to adsorption, the value of A in 313 K did not remarkably increase (Table 4). It was due to that the migration velocity and the contact between glycerol molecules and resin also increased and influence the adsorption of 1,3-PD by occupying adsorption sites. The adsorption of 1,3-PD to the resin was spontaneous and endothermic. The adsorption kinetics was accurately represented by the shell progressive model and indicated that the particle diffusion was the rate-limiting step[19]. Considering decreasing operating cost and simplifying experiments, the temperature was fixed at 298 K. Data was fitted based on Yoon-Nelson kinetic model, the results of simulating parameters shows in Table 4 and Fig. 8. As showed in the Fig. 8, the value of k decreased with the increasing of temperature. The value of xexp by using model and x shared a similar result, indicated that Yoon-Nelson kinetic model can simulate the adsorption process under different temperatures. 3.4 The effect of stack height on adsorption The effect of stack height on adsorption was studied with 10.0, 15.0 and 30.0 cm respectively (Fig. 9A). Experiment condition: flow rate, 1.00 mL min-1, fermentation broth with 1,3-PD,30.0g L-1; stacking height, 10.0, 15.0, 30.0 cm; temperature, 298 K. As showed in Fig. 9A, the slope of the breakthrough curve gradually decreased with the increasing of stack height, indicated that high stack height promoted adsorption. Supplementary analysis shows in Table 5. As showed in Table 5, qtotal and A was low when H was 10.0 cm for the resin quantity and the active sites of fixed bed decreased. The MTZ (8.44 cm) was nearly as the whole bed height, showing that the relative low efficiency of the adsorption. The parameter was small owing to reasons above, placing competing adsorption between 1,3-PD and glycerol, also the shorter contact time between resin and solution for decreasing height. Comparably, the values of breakthrough parameters when H equaled to 30.0 cm were fine: the maximum of q total attained 2.13 g, which was significantly higher than other values and the value of A was also maximum. The main reason was the increasing stack height resulted in the increasing of contacting time between resin and adsorption sites for better adsorption. On the other hand, with the adding of resin in adsorption bed, improved the capability of adsorption to 1,3-PD (te increased, the slope of breakthrough curves, the adsorption of resin increased). The bre akthrough curves in three stack heights showed in Fig. 9B. The adsorption of glycerol is 117, 269 and 388 mg/mg, respectively. Moreover, the increasing of bed height increased the number of theoretical plates in separation degree. Considering the value of tb, te and A , 30.0 cm was chosen for the following experiments. Data fitting based on Yoon-Nelson kinetic model, the results of simulating parameters of the adsorption process in Table 5 and Fig. 10. The value of k decreased with the increasing of stack height and xexp was similar to x, indicating that Yoon-Nelson kinetic model can fit the adsorption in different stack height. M0 (g) is the elution quantity of 1,3-PD, M1 (g) is the quantity of glycerol The effect of ethanol concentration on elution curves was studied with three different ethanol volumes (30%, 50%, 75%), which were showed in Fig 11. As shown in Fig 11, 1,3-PD was the eluted out firstly. The retention time of 1.3-PD and glycerol enlarged in the column were due to the stack height of this fixed bed (30.0 cm). The overlapping of curves between components decreased obviously. It also prolonged the adsorption time between 1.3-PD and glycerol with resin. 1.3-PD and glycerol separated through continuous adsorption and desorption. Resin prefers the adsorption of glycerol to 1,3-PD. Glycerol replaced the 1,3-PD which adsorbed on the resin, caused the glycerol layer enlarged while 1,3-PD layer moved down. Furthermore, ethanol selectively eluted 1,3-PD. Therefore, the 1,3-PD was the firstly been eluted. With the elution of 1,3-PD, the percentage of 1,3-PD in eluent decreased. The ability of the eluent increased so glycerol been eluted down. Besides, when the volume percentage of ethanol increasing from 30% to 75%, both peaks of 1,3-PD and glycerol improved and concentrated, indicating that high concentration of ethanol was advantageous for the collection of components. The elution quantity of 1,3-PD (M0) and elution quantity of glycerol (M1), were calculated according to elution curves and showed in Table 6. As showed in Table 6, the elution rate of 1,3-PD improved from 64.3% to 95.3%, indicating that high concentration ethanol was helpful to the elution of relevant components. Meanwhile, both elution ratio of 1,3-PD and glycerol increased and attained a maximum when ethanol was 75%. The mass ratio of 1,3-PD and glycerol in eluent improved from 6:1 to 14.6:1 in broth, and the concentration of 1,3-PD increased from 85.7% to 93.6%, indicating that both of 1,3-PD and glycerol been divided efficiently. Moreover, with the increasing of ethanol volume percentage, the elution amount of both 1,3-PD and glycerol increased. However, the increasing of elution amount of glycerol was little, which mean the combination force between glycerol and resin was stronger that 75% ethanol could not eluted glycerol down. 100% ethanol was also used for elution and caused severe dehydration of resin. Therefore, 75% ethanol was used as eluent. 3.6 The effect of the elution flow rate The flow rate increasing from 1.00 to 2.00 mL min-1 to investigate the effect of elution flow rate on 1,3-PD and glycerol. The results showed in Fig. 12 and relevant parameters in Table 7. As showed in Fig. 12, increasing flow rate, the elution peak of both 1,3-PD and glycerol decreased. In Table 7, with the increasing of flow rate, the elution of 1,3-PD decreased while that of glycerol improved. Meanwhile, the mass ratio of 1.3-PD to glycerol decreased from 14.6 to 9.88, indicating that increasing elution rate was not favorable to the separation of 1,3-PD and glycerol. Therefore, the elution efficiency and 1,3-PD separation could be improved significantly if slow down elution rate and increasing contact time between eluent and 1,3-PD in the resin. As shown in Fig. 11 and Fig. 12, when the flow rate was 1.00 mL min-1 and the concentration of ethanol is 75%, 1,3-PD and glycerol showed a seemingly separation on the curve: the curve of glycerol appeared from 54 mL which means before that the system only contained 1,3-PD; after 98 mL 1,3-PD and glycerol coexist in the mixture. It is known by calculation that before 54 mL the quantity of 1,3-PD was 1.64 g as a percentage of 80.8%. Mixture collected could return to the fermentation broth for the next adsorption process. 1,3-PD could be acquired by first 54 mL eluent after vacuum distillation. Further resolution of the two substances with the flow rate as 0.500 mL min-1 were studied. The overlapping degree of 1,3-PD and glycerol curves reduced, but could not be completely separated. Meanwhile, the time increased remarkably as 304 min, indicating that although decreasing flow rate was helpful to improve separation efficiency, the production efficiency decreased. Therefore, 1.00 mL min-1 was selected as the best flow rate. 3.7 Purification of the 1,3-propanediol Vacuum distillation, which is energy saving due to the decline of boiling point, is preferred to traditional distillation. 324 ml eluent was collected after six times adsorption and elution separation. No glycerol has been tested by HPLC. The eluent was added into rotary evaporator (vacuum 0.093-0.097 MPa and temperature 60-65 oC) for ethanol recycling. After recycling, the remaining solution transferred to vacuum distillation under Vacuum degree at 0.093-0.097 MPa. Collecting impurity below 129 oC and 1,3-PD fraction between 129-149 oC. The purity of 1,3-PD has been tested by HPLC is 99.2% (sample has been sent to the independent third test institution for detection). 4 Conclusion Recovery and purification of 1,3-PD from complex fermentation broth represents a technological challenge and true bottleneck in the development of a commercially viable bioprocess of this promising bulk chemicals. The present work provides a novel technique for purification of 1,3-propanediol from crude glycerol-based fermentation broth. Separation and purification of 1,3-propanediol was achieved by four simple steps: removal of cells and proteins by chitosan flocculation, decoloration by activated carbon, adsorption by fixed bed cation exchange resin, and vacuum distillation. Furthermore, in order to predict the breakthrough curves and to determine the characteristic parameters of the column, Yoon-Nelson models were applied to the experimental data. Parameters as adsorption capacity at breakthrough time (tb) and saturation time (ts), length of the mass transfer zone (MTZ) were obtained for the different operation conditions used in the adsorption experiments. The characteristic colu mn parameters were calculated for process design. The overall yield of 1,3-propanediol recovery is calculated to be 80.8% with 99.2% of purity. This process, which is simple, fast, and efficient, will promote the commercialization of 1,3-propanediol production.